Please use this identifier to cite or link to this item:
Files in This Item:
There are no files associated with this item.
Title: Valorisation of Brewer's Spent Yeasts' Hydrolysates as High-Value Bioactive Molecules
Authors: San Martin, David; Ibarruri, Jone; Inarra, Bruno; Luengo, Nagore; Ferrer, Jorge; Alvarez-Ossorio, Carmen; Bald, Carlos and Gutierrez, Monica; Zufia, Jaime
Abstract: Brewer's spent yeast (BSY) is produced by the beer industry and has high nutritional value and great potential for producing high-value molecules, such as peptides, for nutraceutical, food and feed applications. In the present research, Flavourzyme(R) and Protamex(R) enzymes were selected for protein hydrolysis based on previous studies. The optimum conditions for the enzymatic hydrolysis were defined by response surface methodology (RSM) by the Box-Behnken design composed of four variables: temperature, pH, enzyme dosage and time. Protein content, hydrolysis degree and the anti-microbial and antioxidant bioactivities of obtained hydrolysates were quantified. Obtained results show that time, enzyme dosage and pH had the highest effect on protein extraction yield (PEY), degree of hydrolysis (DH) and antioxidant activity. Response variables ranged from 13.7 to 29.7\% for PEY, from 6.3 to 35.7\% for DH and from 0.65 to 1.65 g for Trolox equivalent antioxidant capacity. Antimicrobial activity, measured as minimum inhibitory concentration, against Aeromonas salmonicida, Bacillus cereus, Bacillus subtilis and Salmonella enterica, ranged from 6.25 to 50 mg/mL. Antioxidant and antimicrobial activity showed the potential use of BSY hydrolysates as an ingredient for functional foods.
Keywords: food waste; circular economy; nutraceutical; food; feed; protein; anti-microbial bioactivity; antioxidant bioactivities; ANTIMICROBIAL PEPTIDES; ANTIOXIDANT PROPERTIES; BETA-GLUCANS; OPTIMIZATION; EXTRACTS; GENERATION; PRODUCTS; CRAFT; WASTE; ACIDS
Issue Date: 2021
Publisher: MDPI
Type: Article
DOI: 10.3390/su13126520
E-ISSN: 2071-1050
Funder: LIFE European Environment Programme [LIFE16ENV/ES/000160]
Appears in Publication types:Artículos científicos

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.