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dc.contributor.authorGarg, Ritu
dc.contributor.authorPerez, Rebeca
dc.contributor.authorMaldener, Iris
dc.date.accessioned2023-10-04T10:45:04Z-
dc.date.available2023-10-04T10:45:04Z-
dc.date.issued2022
dc.identifierWOS:000774477200015
dc.identifier.urihttp://dspace.azti.es/handle/24689/1582-
dc.description.abstractSeveral filamentous cyanobacteria like Nostoc differentiate specialized cells in response to changes in environmental factors, such as low light or nutrient starvation. These specialized cells are termed heterocysts and akinetes. Under conditions of nitrogen limitation, nitrogen-fixing heterocysts form in a semi-regular pattern and provide the filament with organic nitrogen compounds. Akinetes are spore-like dormant cells, which allow survival during adverse unfavorable conditions. Both cell types possess multilayered thick envelopes mainly composed of an outermost polysaccharide layer and inner layers of glycolipids, that are important for stress adaptation. To study these envelope glycolipids, a method for the isolation, separation and analysis of lipids from heterocysts and akinetes is essential. The present protocol describes a method involving the extraction of lipids from cyanobacteria using solvents and their separation and visualization on silica plates, to render analysis simple and easy. This protocol is relevant for studying mutants that are defective in glycolipid layer formation and for the comparison of glycolipid composition of heterocysts and akinetes under different environmental stresses.
dc.language.isoEnglish
dc.publisherBIO-PROTOCOL
dc.subjectCyanobacteria
dc.subjectHeterocyst
dc.subjectAkinete
dc.subjectEnvelope
dc.subjectGlycolipids
dc.subjectThin layer chromatography
dc.subjectENVELOPE
dc.titleAnalysis of Heterocyst and Akinete Specific Glycolipids in Cyanobacteria Using Thin-layer Chromatography
dc.typeArticle
dc.identifier.journalBIO-PROTOCOL
dc.format.volume12
dc.contributor.funderDeutsche Forschungsgemeinschaft (DFG, German Research Foundation) [GRK1708]
dc.identifier.e-issn2331-8325
dc.identifier.doi10.21769/BioProtoc.4355
Appears in Publication types:Artículos científicos



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