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dc.contributor.authorPawlowski, J.-
dc.contributor.authorBruce, K.-
dc.contributor.authorPanksep, K.-
dc.contributor.authorAguirre, I, F.; Amalfitano, S.-
dc.contributor.authorApotheloz-Perret-Gentil, L.-
dc.contributor.authorBaussant, T.; Bouchez, A.-
dc.contributor.authorCarugati, L.-
dc.contributor.authorCermakova, K.-
dc.contributor.authorCordier, T.; Corinaldesi, C.-
dc.contributor.authorCosta, F. O.-
dc.contributor.authorDanovaro, R.-
dc.contributor.authorDell'Anno, A.; Duarte, S.-
dc.contributor.authorEisendle, U.-
dc.contributor.authorFerrari, B. J. D.-
dc.contributor.authorFrontalini, F.; Fruehe, L.-
dc.contributor.authorHaegerbaeumer, A.-
dc.contributor.authorKisand, V-
dc.contributor.authorKrolicka, A.;-
dc.contributor.authorLanzen, Anders-
dc.contributor.authorLeese, F.; Lejzerowicz, F.-
dc.contributor.authorLyautey, E.-
dc.contributor.authorMacek, I-
dc.contributor.authorSagova-Mareckova, M.-
dc.contributor.authorPearman, J. K.-
dc.contributor.authorPochon, X.-
dc.contributor.authorStoeck, T.-
dc.contributor.authorVivien, R.-
dc.contributor.authorWeigand, A.-
dc.contributor.authorFazi, S.-
dc.date.accessioned2023-10-04T10:45:11Z-
dc.date.available2023-10-04T10:45:11Z-
dc.date.issued2022-
dc.identifierWOS:000820292400004-
dc.identifier.issn0048-9697-
dc.identifier.urihttp://dspace.azti.es/handle/24689/1650-
dc.description.abstractEnvironmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for identification of whole communities within a targeted group) is revolutionizing the field of aquatic biomonitoring. To date, most metabarcoding studies aiming to assess the ecological status of aquatic ecosystems have focused on water eDNA and macroinvertebrate bulk samples. However, the eDNA metabarcoding has also been applied to soft sediment samples, mainly for assessing microbial or meiofaunal biota. Compared to classical methodologies based on manual sorting and morphological identification of benthic taxa, eDNA metabarcoding offers potentially important advantages for assessing the environmental quality of sediments. The methods and protocols utilized for sediment eDNA metabarcoding can vary considerably among studies, and standardization efforts are needed to improve their robustness, comparability and use within regulatory frameworks. Here, we review the available information on eDNA metabarcoding applied to sediment samples, with a focus on sampling, preservation, and DNA extraction steps. We discuss challenges specific to sediment eDNA analysis, including the variety of different sources and states of eDNA and its persistence in the sediment. This paper aims to identify good-practice strategies and facilitate method harmonization for routine use of sediment eDNA in future benthic monitoring.-
dc.language.isoEnglish-
dc.publisherELSEVIER-
dc.subjectEnvironmental DNA-
dc.subjectSediments-
dc.subjectMetabarcoding-
dc.subjectMonitoring-
dc.subjectAquatic ecosystems-
dc.subjectECOLOGICAL QUALITY STATUS-
dc.subjectFRESH-WATER SEDIMENTS-
dc.subjectCOMMUNITY-BASED INDEX-
dc.subjectMARINE-SEDIMENTS-
dc.subjectBACTERIAL COMMUNITY-
dc.subjectEXTRACELLULAR DNA-
dc.subjectMICROBIAL DNA-
dc.subjectOFFSHORE OIL-
dc.subjectEUKARYOTIC DIVERSITY-
dc.subjectINTRACELLULAR DNA-
dc.titleEnvironmental DNA metabarcoding for benthic monitoring: A review of sediment sampling and DNA extraction methods-
dc.typeReview-
dc.identifier.journalSCIENCE OF THE TOTAL ENVIRONMENT-
dc.format.volume818-
dc.contributor.funderEuropean Cooperation in Science and Technology (COST) [CA15219]-
dc.contributor.funderMinistry of University and Research of Italy (MUR) [2017EKFA98]-
dc.contributor.funderIstituto Superiore per la Protezione e la Ricerca Ambientale (ISPRA)-
dc.contributor.funderSwiss National Science Foundation [31003A\_179125]-
dc.contributor.funderEarly Postdoc. Mobility grant [P2GEP3\_171829]-
dc.contributor.funderMinistry of Education, Youth and Sports of the Czech Republic, European Regional Development Fund [CZ.02.1.01/0.0/0.0/16\_019/0000845, LTC 20063]-
dc.contributor.funderEuropean Regional Development Fund within National Programme for Addressing Socio-Economic Challenges through RD [L190003VLVI RITA1/02-60-05]-
dc.contributor.funderEstonian University of Life Sciences [P190250PKKH]-
dc.contributor.funderSlovenian Research Agency (ARRS) [P4-0085]-
dc.contributor.funderPortuguese Foundation for Science and Technology (FCT) [CEECIND/00667/2017]-
dc.contributor.funderPON AIM [1854833, 407]-
dc.contributor.funderInstitute of Technology, University of Tartu-
dc.contributor.funder[PUT1389]-
dc.contributor.funderSwiss National Science Foundation (SNF) [P2GEP3\_171829] Funding Source: Swiss National Science Foundation (SNF)-
dc.identifier.e-issn1879-1026-
dc.identifier.doi10.1016/j.scitotenv.2021.151783-
Appears in Publication types:Artículos científicos



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