Please use this identifier to cite or link to this item: http://dspace.azti.es/handle/24689/1698
Files in This Item:
There are no files associated with this item.
Full metadata record
DC FieldValueLanguage
dc.contributor.authorPawar, Sachin S.-
dc.contributor.authorKurade, Nitin P.-
dc.contributor.authorBhendarkar, Mukesh P.-
dc.contributor.authorBhosale, Shivaji V.; Nirmale, Avinash V.-
dc.contributor.authorKochewad, Sanjiv A.-
dc.date.accessioned2024-03-12T11:49:13Z-
dc.date.available2024-03-12T11:49:13Z-
dc.date.issued2023-
dc.identifierWOS:000971624900001-
dc.identifier.citationANIMAL BIOTECHNOLOGY, 2023, 34, 5168-5172-
dc.identifier.issn1049-5398-
dc.identifier.urihttp://dspace.azti.es/handle/24689/1698-
dc.description.abstractThe present study was conducted to assess the effect of exposure to heat stress on the HSP70 gene expression pattern ex vivo in chickens. The adult healthy birds (n = 15) grouped into three replicates of n = 5 each were used to isolate peripheral blood mononuclear cells (PBMCs). The PBMCs were subjected to heat stress at 42 degrees C for 1 h, and cells without heat stress (NHS) were taken as control. The cells were seeded in 24 well plates and incubated in a humidified incubator at 37 degrees C under 5\% CO2 for recovery. HSP70 expression kinetics were evaluated at 0, 2, 4, 6, and 8 h of the recovery period. Compared with NHS, the expression pattern of HSP70 was upregulated gradually from 0 to 4 h with peak (p < 0.05) expression recorded at 4 h of recovery time. mRNA expression of HSP70 escalated in a time-dependent manner from 0 to 4 h of heat exposure and thereafter exhibited a gradually decreasing pattern till 8 h of the recovery period. The findings from this study highlight the protective role of HSP70 against the deleterious effects of heat stress in chicken PBMCs. Further, the study demonstrates the possible use of PBMCs as a cellular system in assessing the heat stress effect in chickens ex vivo.-
dc.language.isoEnglish-
dc.publisherTAYLOR \& FRANCIS INC-
dc.subjectThermal stress-
dc.subjectchicken-
dc.subjectHSP70-
dc.subjectPBMC-
dc.subjectMESSENGER-RNA EXPRESSION-
dc.subjectSHOCK PROTEINS-
dc.subjectDIFFERENTIAL EXPRESSION-
dc.subjectBLOOD-
dc.subjectANTIOXIDANT-
dc.subjectTEMPERATURE-
dc.subjectIMPACT-
dc.subjectCATTLE-
dc.titleModulation of heat shock protein 70 (HSP70) gene expression <i>ex vivo</i> in response to heat stress in chicken-
dc.typeArticle; Early Access-
dc.identifier.journalANIMAL BIOTECHNOLOGY-
dc.format.page5168-5172-
dc.format.volume34-
dc.contributor.funderNational Institute of Abiotic Stress Management, Indian Council of Agricultural Research-
dc.identifier.e-issn1532-2378-
dc.identifier.doi10.1080/10495398.2023.2200422-
Appears in Publication types:Artículos científicos



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.