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Title: Characterization of the heteropolysaccharides produced by Liquorilactobacillus sicerae CUPV261 and Secundilactobacillus collinoides CUPV237 isolated from cider{*
Authors: Puertas, Ana Isabel; Llamas-Arriba, Ma Goretti; Etxebeste, Oier; Berregi, Inaki; Pardo, Miguel Angel; Prieto, Alicia; Lopez, Paloma; Duenas, Teresa
Abstract: Some lactic acid bacteria (LAB) strains isolated from alcoholic beverages are able to produce exopolysaccharides (EPS). The present work focuses on the physico-chemical characterization of the heteropolysaccharides (HePS) produced by Liquorilactobacillus sicerae CUPV261T (formerly known as Lactobacillus sicerae) and Secundilactobacillus collinoides CUPV237 (formerly known as Lactobacillus collinoides) strains isolated from cider. Genome sequencing and assembly enabled the identification of at least four putative HePS gene clusters in each strain, which correlated with the ability of both strains to secrete EPS. The crude EPS preparation from CUPV261T contained glucose, galactose and rhamnose, and that of CUPV237 was composed of glucose, galactose and Nacetylglucosamine. Both EPS were mixtures of HePS of different composition, with two major soluble components of average molecular weights (Mw) in the range of 106 and 104 g.mol- 1. These HePS were resistant to gastric stress conditions in an in vitro model, and they significantly reduced zebrafish larvae mortality in an in vivo model of inflammatory bowel disease.
Keywords: Lactic acid bacteria; Liquorilactobacillus sicerae; Secundilactobacillus collinoides; Exopolysaccharides; Heteropolysaccharides; Gene clusters; Cider; Zebrafish; LACTIC-ACID BACTERIA; BETA-D-GLUCAN; PEDIOCOCCUS-PARVULUS; OENOCOCCUS-OENI; EXOPOLYSACCHARIDE PRODUCTION; PROBIOTIC PROPERTIES; STRUCTURAL-ANALYSIS; HUMAN ENTEROCYTE; WEB SERVER; PROTEIN
Issue Date: 2023
Publisher: ELSEVIER
Type: Article; Early Access
DOI: 10.1016/j.ijfoodmicro.2023.110199
ISSN: 0168-1605
E-ISSN: 1879-3460
Funder: Spanish Ministry of Science, Inno-vation and Universities [RTI2018-097114-B-I00]
Basque Government [KK-2019/00076, KK-2021/00034, KK-2022/00107, IT1662-22, PIBA\_2020\_1\_0032]
University of the Basque Country [GIU19/014]
Appears in Publication types:Artículos científicos

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