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Title: A real-time PCR approach to detect predation on anchovy and sardine early life stages
Authors: Cuende, Elsa; Mendibil, Inaki; Bachiller, Eneko; Alvarez, Paula; Rodriguez-Ezpeleta, Naiara
Citation: JOURNAL OF SEA RESEARCH, 2017, 130, 204-209
Abstract: Recruitment of sardine (Sardina pilchardus Walbaum, 1792) and anchovy (Engraulis encrasicolus Linnaeus, 1758) is thought to be regulated by predation of their eggs and larvae. Predators of sardine and anchovy can be identified by visual taxonomic identification of stomach contents, but this method is time consuming, tedious and may underestimate predation, especially in small predators such as fish larvae. Alternatively, genetic tools may offer a more cost-effective and accurate alternative. Here, we have developed a multiplex real-time polymerase chain reaction (RT-PCR) assay based on TaqMan probes to simultaneously detect sardine and anchovy remains in gut contents of potential predators. The assay combines previously described and newly generated species-specific primers and probes for anchovy and sardine detection respectively, and allows the detection of 0,001 ng of target DNA (which corresponds to about one hundredth of the total DNA present in a single egg). We applied the method to candidate anchovy and sardine egg predators in the Bay of Biscay, Atlantic Mackerel (Scomber scombrus) larvae. Egg predation observed was limited primarily to those stations where sardine and/or anchovy eggs were present. Our developed assay offers a suitable tool to understand the effects of predation on the survival of anchovy and sardine early life stages.
Keywords: Early life stage mortality; Pelagic fish; Predation; Stomach contents; TaqMan probe; Mackerel larvae; SMALL PELAGIC FISH; MACKEREL SCOMBER-SCOMBRUS; MOLECULAR-IDENTIFICATION; EGG MORTALITY; TAQMAN PROBES; BISCAY; BAY; LARVAE; PREY; DIET
Issue Date: 2017
Type: Article; Proceedings Paper
Language: English
DOI: 10.1016/j.seares.2017.06.009
ISSN: 1385-1101
E-ISSN: 1873-1414
Funder: Department of Agriculture, Fisheries and Food (Basque Government) through the project ``GENOPES: De los genes a la gestion pesquera��
Department of Agriculture, Fisheries and Food (Basque Government) through the project ``PROCES: Procesos de Reclutamiento de Poblaciones Explotadas��
Department of Education, Language policy and Culture (Basque Government)
Marine Research Division (AZTI-Tecnalia) [814]
Appears in Publication types:Artículos científicos

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