Baseline levels of environmental genotoxicity and potential confounding factors using common sole (Solea solea) as sentinel organism

Abstract

Micronuclei (MN) and nuclear buds (NB) frequency was assessed monthly over a one-year period in erythrocytes of common soles (Solea solea) from the Basque continental shelf, a minor disturbed area, in order to determine baseline levels and potential biotic and abiotic confounding factors within biomonitoring purposes. Both genotoxic parameters presented seasonal variations, although only NB frequency was positively correlated with environmental variables (i.e. water temperature and salinity). On the contrary, MN and NB were not affected by age and gender. Therefore, samples of combined age and gender can be used for biomonitoring genotoxic effects, although sampling period together with water temperature and salinity should be thoroughly considered for NB. Overall, these findings are the first attempt to establish baseline MN (0.10-0.78 parts per thousand) and NB (0.13-0.82 parts per thousand) frequencies in common sole from Basque marine waters, which are useful for upcoming data comparisons and integration within genotoxicity evaluation procedures of similar marine environments under biomonitoring scopes.